Western blot analysis of extracts of various cell lines, using Zyxin Rabbit mAb (A2298) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Zyxin Rabbit mAb
Catalog No:AB-A2298
Manufacturer:ABclonal
Synonyms:ESP-2, HED-2
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | Zyxin Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform. [provided by RefSeq, Jul 2008] |
Immunogen: | A synthesized peptide derived from human Zyxin |
Gene ID: | 7791 |
Swiss prot: | Q15942 |
Molecular weight: | 78kDa |
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