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Western blot analysis of extracts of various cell lines, using ZFX antibody (A16444) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
ZFX Rabbit pAb
Catalog No：AB-A16444
Manufacturer：ABclonal
Synonyms：ZFX;ZNF926
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | ZFX Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene on the X chromosome is structurally similar to a related gene on the Y chromosome. It encodes a member of the krueppel C2H2-type zinc-finger protein family. The full-length protein contains an acidic transcriptional activation domain (AD), a nuclear localization sequence (NLS) and a DNA binding domain (DBD) consisting of 13 C2H2-type zinc fingers. Studies in mouse embryonic and adult hematopoietic stem cells showed that this gene was required as a transcriptional regulator for self-renewal of both stem cell types, but it was dispensable for growth and differentiation of their progeny. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, but the full-length nature of some variants has not been determined. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 180-380 of human ZFX (NP_003401.2). |
| Gene ID: | 7543 |
| Swiss prot: | P17010 |
| Molecular weight: | 66kDa/90kDa/94kDa |
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