Western blot analysis of extracts of various cell lines, using WNT3 antibody (A9328) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
WNT3 Rabbit pAb
Catalog No:AB-A9328
Manufacturer:ABclonal
Synonyms:WNT3;INT4;TETAMS
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | WNT3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 166-355 of human WNT3 (NP_110380.1). |
Gene ID: | 7473 |
Swiss prot: | P56703 |
Molecular weight: | 39kDa |
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