Western blot analysis of extracts of various cell lines, using WASF1 antibody (A14624) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
WASF1 Rabbit pAb
Catalog No:AB-A14624
Manufacturer:ABclonal
Synonyms:WASF1;SCAR1;WAVE;WAVE1
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | WASF1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human WASF1 (NP_001020107.1). |
Gene ID: | 8936 |
Swiss prot: | Q92558 |
Molecular weight: | 61kDa |
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