Western blot analysis of extracts of various cell lines, using VPS37A antibody (A14159) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
VPS37A Rabbit pAb
Catalog No:AB-A14159
Manufacturer:ABclonal
Synonyms:VPS37A;HCRP1;PQBP2;SPG53
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | VPS37A Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene belongs to the VPS37 family, and encodes a component of the ESCRT-I (endosomal sorting complex required for transport I) protein complex, required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies. Expression of this gene is downregulated in hepatocellular carcinoma, and mutations in this gene are associated with autosomal recessive spastic paraplegia-53. A related pseudogene has been identified on chromosome 5. Alternatively spliced transcript variants have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 224-397 of human VPS37A (NP_689628.2). |
Gene ID: | 137492 |
Swiss prot: | Q8NEZ2 |
Molecular weight: | 20kDa/41kDa/44kDa |
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