Western blot analysis of extracts of HUVEC cells, using VCAM1 antibody (A0279) at 1:1000 dilution.HUVEC cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Western blot analysis of extracts of Rat lung, using VCAM1 antibody (A0279) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
VCAM1 Rabbit pAb
Catalog No:AB-A0279
Manufacturer:ABclonal
Synonyms:CD106;INCAM-100;VCAM1
Applications:WB,IHC,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | VCAM1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human VCAM1 (NP_001069.1). |
Gene ID: | 7412 |
Swiss prot: | P19320 |
Molecular weight: | 71kDa/74kDa/81kDa |
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