Western blot analysis of extracts of various cell lines, using USP5 antibody (A4202) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
USP5 Rabbit pAb
Catalog No:AB-A4202
Manufacturer:ABclonal
Synonyms:USP5;ISOT
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | USP5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP5 (NP_003472.2). |
Gene ID: | 8078 |
Swiss prot: | P45974 |
Molecular weight: | 93kDa/95kDa |
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