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Western blot analysis of extracts of K-562 cells, using USP3 antibody (A15769) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
USP3 Rabbit pAb
Catalog No：AB-A15769
Manufacturer：ABclonal
Synonyms：USP3;SIH003;UBP
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | USP3 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP3 (ubiquitin specific peptidase 3), also known as UBP or SIH003, is a 520 amino acid protein that contains one UBP-type zinc finger and belongs to the peptidase C19 family. Expressed ubiquitously with highest levels present in pancreas, USP3 catalyzes the conversion of a ubiquitin C-terminal thioester to a free ubiquitin and a thiol. The gene encoding USP3 maps to human chromosome 15, which houses over 700 genes and comprises nearly 3% of the human genome. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 321-520 of human USP3 (NP_006528.2). |
| Gene ID: | 9960 |
| Swiss prot: | Q9Y6I4 |
| Molecular weight: | 53kDa/58kDa |
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