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Western blot analysis of extracts of various cell lines, using UPF3B antibody (A15184) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
UPF3B Rabbit pAb
Catalog No：AB-A15184
Manufacturer：ABclonal
Synonyms：UPF3B;HUPF3B;MRX62;MRXS14;RENT3B;UPF3BP1;UPF3BP2;UPF3BP3;UPF3X;Upf3p-X
Applications：WB
Reactivity：Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | UPF3B Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human UPF3B (NP_075386.1). |
| Gene ID: | 65109 |
| Swiss prot: | Q9BZI7 |
| Molecular weight: | 56kDa/57kDa |
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