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Western blot analysis of extracts of HepG2 cells, using UPF1/RENT1 antibody (A13541) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).
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Immunohistochemistry of paraffin-embedded human liver cancer using UPF1/RENT1 antibody (A13541) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using UPF1/RENT1 antibody (A13541) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using UPF1/RENT1 antibody (A13541) at dilution of 1:100 (40x lens).
UPF1/RENT1 Rabbit pAb
Catalog No：AB-A13541
Manufacturer：ABclonal
Synonyms：UPF1;HUPF1;NORF1;RENT1;pNORF1;smg-2
Applications：WB,IHC
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | UPF1/RENT1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 270-370 of human UPF1/RENT1 (NP_002902.2). |
| Gene ID: | 5976 |
| Swiss prot: | Q92900 |
| Molecular weight: | 123kDa/124kDa |
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