Western blot analysis of extracts of various cell lines, using UMPS antibody (A13251) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
UMPS Rabbit pAb
Catalog No:AB-A13251
Manufacturer:ABclonal
Synonyms:UMPS;OPRT
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | UMPS Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a uridine 5′-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5′-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5′-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-260 of human UMPS (NP_000364.1). |
Gene ID: | 7372 |
Swiss prot: | P11172 |
Molecular weight: | 22kDa/33kDa/42kDa/52kDa |
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