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Western blot analysis of extracts of various cell lines, using UCP3 antibody (A1532) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
UCP3 Rabbit pAb
Catalog No：AB-A1532
Manufacturer：ABclonal
Synonyms：UCP3;SLC25A9
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | UCP3 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene’s protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human UCP3 (NP_073714.1). |
| Gene ID: | 7352 |
| Swiss prot: | P55916 |
| Molecular weight: | 22kDa/29kDa/34kDa |
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