Western blot analysis of extracts of mouse spleen, using UCP2 antibody (A4178) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
UCP2 Rabbit pAb
Catalog No:AB-A4178
Manufacturer:ABclonal
Synonyms:UCP2;BMIQ4;SLC25A8;UCPH
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | UCP2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5′-UCP3-UCP2-3′. |
Immunogen: | A synthetic peptide of human UCP2 |
Gene ID: | 7351 |
Swiss prot: | P55851 |
Molecular weight: | 33kDa |
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