Western blot analysis of extracts of various cell lines, using Ubiquityl-Histone H2A-K119 Rabbit pAb (A17952) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Ubiquityl-Histone H2A-K119 Rabbit pAb
Catalog No:AB-A17952
Manufacturer:ABclonal
Synonyms:HIST1H2AB
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Ubiquityl-Histone H2A-K119 Rabbit pAb |
Category: | Other Modified Antibodies |
Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.H2A clustered histone (H2AC) consists of several H2A family members, which function as replication-dependent histone proteins. |
Immunogen: | A synthetic peptide of human HIST1H2AB. |
Gene ID: | 3012/8335 |
Swiss prot: | P04908 |
Molecular weight: |
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