Western blot analysis of extracts of various cell lines, using UBE4B antibody (A17609) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
UBE4B Rabbit pAb
Catalog No:AB-A17609
Manufacturer:ABclonal
Synonyms:E4;HDNB1;UBOX3;UFD2;UFD2A;UBE4B
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | UBE4B Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 130-250 of human UBE4B (NP_006039.2). |
Gene ID: | 10277 |
Swiss prot: | O95155 |
Molecular weight: | 121kDa/133kDa/146kDa/151kDa |
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