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Western blot analysis of extracts of various cell lines, using UBE4A antibody (A3354) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using UBE4A Rabbit pAb (A3354) at dilution of 1:100 (40x lens).
UBE4A Rabbit pAb
Catalog No：AB-A3354
Manufacturer：ABclonal
Synonyms：UBE4A;E4;UBOX2;UFD2
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | UBE4A Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn’s disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 797-1066 of human UBE4A (NP_001191006.1). |
| Gene ID: | 9354 |
| Swiss prot: | Q14139 |
| Molecular weight: | 122kDa/123kDa |
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