Western blot analysis of extracts of various cell lines, using Ube2N/Ubc13 antibody (A10543) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
Ube2N/Ubc13 Rabbit pAb
Catalog No:AB-A10543
Manufacturer:ABclonal
Synonyms:UBE2N;HEL-S-71;UBC13;UBCHBEN;UbcH-ben;UbcH13
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Ube2N/Ubc13 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human Ube2N/Ubc13 (NP_003339.1). |
Gene ID: | 7334 |
Swiss prot: | P61088 |
Molecular weight: | 17kDa |
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