Western blot analysis of extracts of various cell lines, using UBE2L3 antibody (A4175) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
UBE2L3 Rabbit pAb
Catalog No:AB-A4175
Manufacturer:ABclonal
Synonyms:UBE2L3;E2-F1;L-UBC;UBCH7;UbcM4
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | UBE2L3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 73-212 of human UBE2L3 (NP_001243284.1). |
Gene ID: | 7332 |
Swiss prot: | P68036 |
Molecular weight: | 14kDa/17kDa/24kDa |
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