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Western blot analysis of extracts of various cell lines, using UBE2D3 antibody (A14640) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
UBE2D3 Rabbit pAb
Catalog No：AB-A14640
Manufacturer：ABclonal
Synonyms：UBE2D3;E2(17)KB3;UBC4/5;UBCH5C
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | UBE2D3 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE2D3 (NP_003331.1). |
| Gene ID: | 7323 |
| Swiss prot: | P61077 |
| Molecular weight: | 16kDa |
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