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Western blot analysis of extracts of various cell lines, using UBE2D2 antibody (A10818) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
UBE2D2 Rabbit pAb
Catalog No：AB-A10818
Manufacturer：ABclonal
Synonyms：UBE2D2;E2(17)KB2;PUBC1;UBC4;UBC4/5;UBCH4;UBCH5B
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | UBE2D2 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D2 (NP_003330.1). |
| Gene ID: | 7322 |
| Swiss prot: | P62837 |
| Molecular weight: | 13kDa/16kDa |
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