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Western blot analysis of extracts of various cell lines, using UBE2D1 antibody (A1951) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).
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Immunohistochemistry of paraffin-embedded rat brain using UBE2D1 antibody (A1951) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using UBE2D1 antibody (A1951) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using UBE2D1 antibody (A1951) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using UBE2D1 antibody (A1951) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using UBE2D1 antibody (A1951) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using UBE2D1 antibody (A1951) at dilution of 1:100 (40x lens).
UBE2D1 Rabbit pAb
Catalog No：AB-A1951
Manufacturer：ABclonal
Synonyms：UBE2D1;E2(17)KB1;SFT;UBC4/5;UBCH5;UBCH5A
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | UBE2D1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D1 (NP_003329.1). |
| Gene ID: | 7321 |
| Swiss prot: | P51668 |
| Molecular weight: | 16kDa |
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