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Western blot analysis of extracts of various cell lines, using UBA3 antibody (A7504) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded mouse kidney using UBA3 antibody (A7504) at dilution of 1:100 (40x lens).
UBA3 Rabbit pAb
Catalog No：AB-A7504
Manufacturer：ABclonal
Synonyms：UBA3;NAE2;UBE1C;hUBA3
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | UBA3 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 234-463 of human UBA3 (NP_003959.3). |
| Gene ID: | 9039 |
| Swiss prot: | Q8TBC4 |
| Molecular weight: | 50kDa/51kDa |
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