Western blot analysis of extracts of PC-12 cells, using Tyrosine Hydroxylase antibody (A12756) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using Tyrosine Hydroxylase antibody (A12756) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Western blot analysis of extracts of Mouse kidney, using Tyrosine Hydroxylase antibody (A12756) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Tyrosine Hydroxylase Rabbit pAb
Catalog No:AB-A12756
Manufacturer:ABclonal
Synonyms:DYT14;DYT5b;TYH;TH
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Tyrosine Hydroxylase Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human Tyrosine Hydroxylase (NP_000351.2). |
Gene ID: | 7054 |
Swiss prot: | P07101 |
Molecular weight: | 44kDa/45kDa/55kDa/56kDa/58kDa |
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