Western blot analysis of extracts of various cell lines, using TRPM5 antibody (A12365) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
TRPM5 Rabbit pAb
Catalog No:AB-A12365
Manufacturer:ABclonal
Synonyms:TRPM5;LTRPC5;MTR1
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | TRPM5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the transient receptor potential (TRP) protein family, which is a diverse group of proteins with structural features typical of ion channels. This protein plays an important role in taste transduction, and has characteristics of a calcium-activated, non-selective cation channel that carries Na+, K+, and Cs+ ions equally well, but not Ca(2+) ions. It is activated by lower concentrations of intracellular Ca(2+), and inhibited by higher concentrations. It is also a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. This gene is located within the Beckwith-Wiedemann syndrome critical region-1 on chromosome 11p15.5, and has been shown to be imprinted, with exclusive expression from the paternal allele. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1026-1165 of human TRPM5 (NP_055370.1). |
Gene ID: | 29850 |
Swiss prot: | Q9NZQ8 |
Molecular weight: | 97kDa/131kDa |
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