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Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K64 antibody (A7259) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 150s.
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Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K64 antibody (A7259) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 120s.
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Immunohistochemistry of paraffin-embedded human breast using TriMethyl-Histone H3-K64 antibody (A7259) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using TriMethyl-Histone H3-K64 antibody (A7259) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K64 antibody (A7259) at dilution of 1:100 (40x lens).
TriMethyl-Histone H3-K64 Rabbit pAb
Catalog No：AB-A7259
Manufacturer：ABclonal
Synonyms：H3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat,Other (Wide Range)
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | TriMethyl-Histone H3-K64 Rabbit pAb |
| Category: | Methylated Antibodies |
| Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
| Immunogen: | A synthetic trimethylated peptide around K64 of human Histone H3 (NP_003520.1). |
| Gene ID: | 8290 |
| Swiss prot: | Q16695 |
| Molecular weight: | 15kDa |
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