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Western blot analysis of extracts of 293T cells, using TriMethyl-Histone H3-K27 antibody (A2363) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human breast cancer using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat ovary using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody (A2363) at dilution of 1:100 (40x lens).
TriMethyl-Histone H3-K27 Rabbit pAb
Catalog No：AB-A2363
Manufacturer：ABclonal
Synonyms：H3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A
Applications：WB,IHC,IF,IP,ChIP,ChIP-seq
Reactivity：Human,Mouse,Rat,Other (Wide Range)
Host：Rabbit
Conjugate：No
€65,00 – €900,00
| Product Name: | TriMethyl-Histone H3-K27 Rabbit pAb |
| Category: | Methylated Antibodies |
| Description: | Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling. |
| Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
| Gene ID: | 8290 |
| Swiss prot: | Q16695 |
| Molecular weight: | 15kDa |
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