Western blot analysis of extracts of various cell lines, using TRIM27 antibody (A6405) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
TRIM27 Rabbit pAb
Catalog No:AB-A6405
Manufacturer:ABclonal
Synonyms:TRIM27;RFP;RNF76
Applications:WB,IHC,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | TRIM27 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 214-513 of human TRIM27 (NP_006501.1). |
Gene ID: | 5987 |
Swiss prot: | P14373 |
Molecular weight: | 41kDa/58kDa |
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