Western blot analysis of extracts of various cell lines, using TRAF4 antibody (A7047) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
TRAF4 Rabbit pAb
Catalog No:AB-A7047
Manufacturer:ABclonal
Synonyms:TRAF4;CART1;MLN62;RNF83
Applications:WB,IHC,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | TRAF4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 251-470 of human TRAF4 (NP_004286.2). |
Gene ID: | 9618 |
Swiss prot: | Q9BUZ4 |
Molecular weight: | 22kDa/53kDa |
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