Western blot analysis of extracts of various cell lines, using TPO-R/CD110/c-Mpl Rabbit mAb (A19729) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using TPO-R/CD110/c-Mpl Rabbit mAb (A19729) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
TPO-R/CD110/c-Mpl Rabbit mAb
Catalog No:AB-A19729
Manufacturer:ABclonal
Synonyms:C-MPL, CD110, MPLV, THCYT2, TPOR
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | TPO-R/CD110/c-Mpl Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq, Jul 2008] |
Immunogen: | A synthesized peptide derived from human TPO-R/CD110/c-Mpl |
Gene ID: | 4352 |
Swiss prot: | P40238 |
Molecular weight: | 82kDa |
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Catalog No:AB-A0175
Manufacturer:ABclonal
Synonyms:PIM;PIM1;PIM1
Applications:WB
Reactivity:Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
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Manufacturer:ABclonal
Synonyms:ARAF;A-RAF;ARAF1;PKS2;RAFA1
Applications:WB,IF
Reactivity:Human
Host:Rabbit
Conjugate:No
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Manufacturer:ABclonal
Synonyms:FOXO3;AF6q21;FKHRL1;FKHRL1P2;FOXO2;FOXO3A
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Catalog No:AB-A0027
Manufacturer:ABclonal
Synonyms:CANDF7;IMD31A;IMD31B;IMD31C;ISGF-3;STAT91;STAT1 alpha;STAT1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
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Manufacturer:ABclonal
Synonyms:10q23del;BZS;CWS1;DEC;GLM2;MHAM;MMAC1;PTEN1;TEP1;PTEN;PTENbeta
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
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Manufacturer:ABclonal
Synonyms:TLR4;ARMD10;CD284;TLR-4;TOLL
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00