Western blot analysis of extracts of NCI-H460 cells, using tPA/tPA/PLAT antibody (A5655) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
tPA/PLAT Rabbit pAb
Catalog No:AB-A5655
Manufacturer:ABclonal
Synonyms:PLAT;T-PA;TPA
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | tPA/PLAT Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes tissue-type plasminogen activator, a secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. The encoded preproprotein is proteolytically processed by plasmin or trypsin to generate heavy and light chains. These chains associate via disulfide linkages to form the heterodimeric enzyme. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, while decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-320 of human tPA/tPA/PLAT (NP_000921.1). |
Gene ID: | 5327 |
Swiss prot: | P00750 |
Molecular weight: | 32kDa/44kDa/57kDa/62kDa |
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