Western blot analysis of extracts of A-549 cells, using TOP1MT antibody (A15559) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60S.
TOP1MT Rabbit pAb
Catalog No:AB-A15559
Manufacturer:ABclonal
Synonyms:TOP1MT
Applications:WB,IHC
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | TOP1MT Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 470-550 of human TOP1MT (NP_443195.1). |
Gene ID: | 116447 |
Swiss prot: | Q969P6 |
Molecular weight: | 58kDa/69kDa |
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