Western blot analysis of extracts of various cell lines, using TNNI3 antibody (A0152) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
TNNI3 Rabbit pAb
Catalog No:AB-A0152
Manufacturer:ABclonal
Synonyms:TNNI3;CMD1FF;CMD2A;CMH7;RCM1;TNNC1;cTnI;troponin I3
Applications:WB,IF
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | TNNI3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human TNNI3 (NP_000354.4). |
Gene ID: | 7137 |
Swiss prot: | P19429 |
Molecular weight: | 24kDa |
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