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Western blot analysis of extracts of various cell lines, using TNF-α antibody (A0277) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded Human colon carcinoma using TNF-α antibody (A0277) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Human breast cancer using TNF-α antibody (A0277) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded Human gastric cancer using TNF-α antibody (A0277) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human colon carcinoma using TNF-α antibody (A0277) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human breast cancer using TNF-α antibody (A0277) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human gastric cancer using TNF-α antibody (A0277) at dilution of 1:100 (40x lens).
TNF-α Rabbit pAb
Catalog No：AB-A0277
Manufacturer：ABclonal
Synonyms：TNF;DIF;TNF-alpha;TNFA;TNFSF2;TNLG1F;TNF alpha
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €1.760,00
| Product Name: | TNF-α Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human TNF (NP_000585.2). |
| Gene ID: | 7124 |
| Swiss prot: | P01375 |
| Molecular weight: | 25kDa |
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