Western blot analysis of extracts of various cell lines, using TIE1 antibody (A3334) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
TIE1 Rabbit pAb
Catalog No:AB-A3334
Manufacturer:ABclonal
Synonyms:TIE1;JTK14;TIE
Applications:WB
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | TIE1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-600 of human TIE1 (NP_005415.1). |
Gene ID: | 7075 |
Swiss prot: | P35590 |
Molecular weight: | 34kDa/40kDa/125kDa |
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