Western blot analysis of extracts of various cell lines, using Thymidine Kinase 1 antibody (A5612) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
Thymidine Kinase 1 Rabbit pAb
Catalog No:AB-A5612
Manufacturer:ABclonal
Synonyms:TK1;TK2
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Thymidine Kinase 1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-234 of human Thymidine Kinase 1 (NP_003249.3). |
Gene ID: | 7083 |
Swiss prot: | P04183 |
Molecular weight: | 25kDa |
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