Western blot analysis of extracts of various cell lines, using THEM4 antibody (A15940) at 1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
THEM4 Rabbit pAb
Catalog No:AB-A15940
Manufacturer:ABclonal
Synonyms:THEM4;CTMP
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | THEM4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Protein kinase B (PKB) is a major downstream target of receptor tyrosine kinases that signal via phosphatidylinositol 3-kinase. Upon cell stimulation, PKB is translocated to the plasma membrane, where it is phosphorylated in the C-terminal regulatory domain. The protein encoded by this gene negatively regulates PKB activity by inhibiting phosphorylation. Transcription of this gene is commonly downregulated in glioblastomas. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 37-150 of human THEM4 (NP_444283.2). |
Gene ID: | 117145 |
Swiss prot: | Q5T1C6 |
Molecular weight: | 27kDa |
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