Western blot analysis of extracts of various cell lines, using TCF4 antibody (A1141) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Western blot analysis of extracts of Mouse brain, using TCF4 antibody (A1141) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
TCF4 Rabbit pAb
Catalog No:AB-A1141
Manufacturer:ABclonal
Synonyms:TCF4;E2-2;FECD3;ITF-2;ITF2;PTHS;SEF-2;SEF2;SEF2-1;SEF2-1A;SEF2-1B;SEF2-1D;TCF-4;bHLHb19;TCF4
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | TCF4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box (‘E-box’) binding site (‘CANNTG’) – a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-500 of human TCF4 (NP_001077431.1). |
Gene ID: | 6925 |
Swiss prot: | P15884 |
Molecular weight: | 48-71kDa |
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