Western blot analysis of extracts of various cell lines, using SUN2 antibody (A16325) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
SUN2 Rabbit pAb
Catalog No:AB-A16325
Manufacturer:ABclonal
Synonyms:SUN2;UNC84B
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SUN2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | SUN1 (MIM 607723) and SUN2 are inner nuclear membrane (INM) proteins that play a major role in nuclear-cytoplasmic connection by formation of a 'bridge' across the nuclear envelope, known as the LINC complex, via interaction with the conserved luminal KASH domain of nesprins (e.g., SYNE1; MIM 608441) located in the outer nuclear membrane (ONM). The LINC complex provides a direct connection between the nuclear lamina and the cytoskeleton, which contributes to nuclear positioning and cellular rigidity (summary by Haque et al., 2010 [PubMed 19933576]). |
Immunogen: | Recombinant protein of human SUN2 |
Gene ID: | 25777 |
Swiss prot: | Q9UH99 |
Molecular weight: | 79kDa/80kDa/82kDa |
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