StuI
Catalog No:AB-RK21162
Manufacturer:ABclonal
Applications:Restriction Endonucleases
€38,00 – €155,00
Description
3’…TCC/GGA…5′
Product Components
Components | Cat. No. |
---|---|
StuI (20, 000 U/ml) | AB-RM21662 |
10X Buffer CutU | AB-RM20102 |
Product Information
Featured | Specification | ||||||
---|---|---|---|---|---|---|---|
Applications | Restriction Enzyme Digestion | ||||||
Unit Definition | One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA fragments in 1 hour at 37°C in a total reaction volume of 50 µl. | ||||||
Storage Conditions | 10 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, pH 7.4 @ 25°C | ||||||
Storage Temperature | -20°C | ||||||
Reaction Conditions | 1X Buffer CutU. Incubate at 37°C. | ||||||
Reaction Buffer or Mix | 1X Buffer CutU: 20 mM Tris-acetate,10 mM Magnesium acetate,50 mM Potassium acetate,100 µg/ml BSA,pH 7.9 @25℃ |
||||||
Heat Inactivation | 80°C for 20 min | ||||||
Methylation Sensitivity |
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