Western blot analysis of extracts of various cell lines, using SPRY2 antibody (A1855) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
SPRY2 Rabbit pAb
Catalog No:AB-A1855
Manufacturer:ABclonal
Synonyms:SPRY2;IGAN3;hSPRY2
Applications:WB,IHC
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SPRY2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SPRY2 (NP_005833.1). |
Gene ID: | 10253 |
Swiss prot: | O43597 |
Molecular weight: | 34kDa |
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