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Western blot analysis of extracts of A375 cells, using SPARC antibody (A14494) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
SPARC Rabbit pAb
Catalog No：AB-A14494
Manufacturer：ABclonal
Synonyms：SPARC;BM-40;OI17
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | SPARC Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1). |
| Gene ID: | 6678 |
| Swiss prot: | P09486 |
| Molecular weight: | 34kDa |
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