Western blot analysis of extracts of various cell lines, using SP1 antibody (A14662) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
SP1 Rabbit pAb
Catalog No:AB-A14662
Manufacturer:ABclonal
Synonyms:SP1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SP1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 420-620 of human SP1 (NP_612482.2). |
Gene ID: | 6667 |
Swiss prot: | P08047 |
Molecular weight: | 75kDa/79kDa/80kDa |
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