Western blot analysis of extracts of RAW264.7 cells, using SOCS3 antibody (A0694) at 1:500 dilution.Raw264.7 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
SOCS3 Rabbit pAb
Catalog No:AB-A0694
Manufacturer:ABclonal
Synonyms:SOCS3;ATOD4;CIS3;Cish3;SOCS-3;SSI-3;SSI3
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SOCS3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human SOCS3 (NP_003946.3). |
Gene ID: | 9021 |
Swiss prot: | O14543 |
Molecular weight: | 24kDa |
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