Western blot analysis of extracts of A-549 cells, using SMPD4 antibody (A15473) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
SMPD4 Rabbit pAb
Catalog No:AB-A15473
Manufacturer:ABclonal
Synonyms:SMPD4;NET13;NSMASE-3;NSMASE3
Applications:WB,IF
Reactivity:Human,Mouse,
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SMPD4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a sphingomyelinase that catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. This gene is activated by DNA damage, cellular stress, and tumor necrosis factor, but it is downregulated by wild-type p53. The encoded protein localizes to the endoplasmic reticulum and Golgi network. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 600-800 of human SMPD4 (NP_060421.2). |
Gene ID: | 55627 |
Swiss prot: | Q9NXE4 |
Molecular weight: | 44kDa, 56-69kDa, 83-98kDa |
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