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Western blot analysis of extracts of various cell lines, using Smad5 antibody (A14023) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human breast cancer using SMAD5 antibody (A14023) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat ovary using SMAD5 antibody (A14023) at dilution of 1:200 (40x lens).
Smad5 Rabbit pAb
Catalog No：AB-A14023
Manufacturer：ABclonal
Synonyms：DWFC;JV5-1;MADH5;SMAD5
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Smad5 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 186-465 of human Smad5 (NP_001001420.1). |
| Gene ID: | 4090 |
| Swiss prot: | Q99717 |
| Molecular weight: | 52kDa |
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