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Western blot analysis of extracts of U-251MG cells, using Smad3 antibody (A11388) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Smad3 Rabbit pAb
Catalog No：AB-A11388
Manufacturer：ABclonal
Synonyms：HSPC193;HsT17436;JV15-2;LDS1C;LDS3;MADH3;SMAD3
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Smad3 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene ‘mothers against decapentaplegic’ (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human Smad3 (NP_005893.1). |
| Gene ID: | 4088 |
| Swiss prot: | P84022 |
| Molecular weight: | 25kDa/35kDa/43kDa/48kDa |
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