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Western blot analysis of extracts of Jurkat cells, using Smad1 Rabbit pAb (A1101) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
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Western blot analysis of extracts of C6 cells, using Smad1 antibody (A1101) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human breast cancer using Smad1 Rabbit pAb (A1101) at dilution of 1:50 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using Smad1 Rabbit pAb (A1101) at dilution of 1:50 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using Smad1 Rabbit pAb (A1101) at dilution of 1:50 (40x lens).
Smad1 Rabbit pAb
Catalog No：AB-A1101
Manufacturer：ABclonal
Synonyms：BSP-1;BSP1;JV4-1;JV41;MADH1;MADR1;Smad1;SMAD1
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Smad1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene ‘mothers against decapentaplegic’ (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human Smad1 (NP_001003688.1). |
| Gene ID: | 4086 |
| Swiss prot: | Q15797 |
| Molecular weight: | 15kDa/52kDa |
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