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Western blot analysis of extracts of various cell lines, using SLC8A1 antibody (A5583) at 1:5000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts of Mouse brain , using SLC8A1 antibody (A5583) at 1:5000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using SLC8A1 Rabbit pAb (A5583) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using SLC8A1 Rabbit pAb (A5583) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat stomach using SLC8A1 Rabbit pAb (A5583) at dilution of 1:100 (40x lens).
SLC8A1 Rabbit pAb
Catalog No：AB-A5583
Manufacturer：ABclonal
Synonyms：SLC8A1;NCX1
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | SLC8A1 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | In cardiac myocytes, Ca(2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca(2+) concentration during contraction is primarily due to release of Ca(2+) from intracellular stores. However, some Ca(2+) also enters the cell through the sarcolemma (plasma membrane). During relaxation, Ca(2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores, the Ca(2+) that entered across the sarcolemma must be extruded from the cell. The Na(+)-Ca(2+) exchanger is the primary mechanism by which the Ca(2+) is extruded from the cell during relaxation. In the heart, the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-520 of human SLC8A1 (NP_066920.1). |
| Gene ID: | 6546 |
| Swiss prot: | P32418 |
| Molecular weight: | 104kDa/107kDa/108kDa |
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