Western blot analysis of extracts of various cell lines, using SLC12A1 antibody (A12999) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
SLC12A1 Rabbit pAb
Catalog No:AB-A12999
Manufacturer:ABclonal
Synonyms:SLC12A1;BSC1;NKCC2
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | SLC12A1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle’s loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SLC12A1 (NP_000329.2). |
Gene ID: | 6557 |
Swiss prot: | Q13621 |
Molecular weight: | 121kDa |
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