Western blot analysis of extracts of various cell lines, using SF3B3/SAP130 Rabbit mAb (A9624) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
SF3B3/SAP130 Rabbit mAb
Catalog No:AB-A9624
Manufacturer:ABclonal
Synonyms:RSE1, SAP130, SF3b130, STAF130
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | SF3B3/SAP130 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008] |
Immunogen: | A synthesized peptide derived from human SF3B3/SAP130 |
Gene ID: | 23450 |
Swiss prot: | Q15393 |
Molecular weight: | 136kDa |
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